Hotspot

rbcL-accD

LSC · 56627-57426 · 800 bp

Back to Aerides

Pi 0.0106

MarkerSeek score 53.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 800
variable_sites: 17
pi: 0.0106
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GGCTTCAGGGGGTATTCATG	CCACTGGTATGTCCAAGAGC	55.0	55.0	58.0	58.0	1218-1229 bp	1.0	81.2
rbcL-accD_p2	TCTCATTATTGCCGCGACAA	CCACTGGTATGTCCAAGAGC	45.0	55.0	58.0	58.0	1508-1519 bp	1.0	80.9
rbcL-accD_p3	TTGTCTCATTATTGCCGCGA	CCACTGGTATGTCCAAGAGC	45.0	55.0	58.0	58.0	1511-1522 bp	1.0	80.9
rbcL-accD_p4	TACGTATGTCTGGCGGAGAT	CCACTGGTATGTCCAAGAGC	50.0	55.0	58.0	58.0	1396-1407 bp	1.0	80.9
rbcL-accD_p5	GAGCGGTATTTGCCAGAGAA	CCACTGGTATGTCCAAGAGC	50.0	55.0	58.0	58.0	1591-1602 bp	1.0	80.8