Hotspot

petN-psbM

LSC · 29466-30376 · 911 bp

Back to Aerides

Pi 0.0208

MarkerSeek score 71.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 911
variable_sites: 27
pi: 0.0208
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGGACATCATTCGCATGGAT	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.1	57.9	1073-1111 bp	1.0	84.1
petN-psbM_p2	GGATAGGGGACATCATTCGC	CGTTTATTGCTACTGCGCTG	55.0	50.0	57.9	57.9	1079-1117 bp	1.0	84.1
petN-psbM_p3	GGGACATCATTCGCATGGAT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.1	58.3	1016-1054 bp	1.0	83.0
petN-psbM_p4	GGATAGGGGACATCATTCGC	ACGTCAAAACAGTCAGCCAA	55.0	45.0	57.9	58.3	1022-1060 bp	1.0	83.0
petN-psbM_p5	GGGACATCATTCGCATGGAT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.1	58.3	1068-1106 bp	1.0	82.7