Hotspot

trnS-GCU-trnG-GCC

LSC · 8351-9579 · 1229 bp

Back to Aerides

Pi 0.0149
MarkerSeek score 72.00
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1229
variable_sites
25
pi
0.0149
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1186-1298 bp 1.0 57.3
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1185-1297 bp 1.0 57.3
trnS-GCU-trnG-GCC_p3 ATCGGAGAGAGAGGGATTCG ACGAATCACACTTTTACCACT 55.0 38.1 58.1 55.5 1241-1353 bp 1.0 56.5
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 1189-1301 bp 1.0 56.2
trnS-GCU-trnG-GCC_p5 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1188-1300 bp 1.0 56.2