Hotspot

psbK-psbI

LSC · 7512-7980 · 469 bp

Back to Aerides

Pi 0.0131

MarkerSeek score 65.30

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 469
variable_sites: 10
pi: 0.0131
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	GCCCCGGATCGTTAGATAAG	50.0	55.0	57.9	57.6	586-589 bp	1.0	78.4
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGCCCAGGATTAC	50.0	50.0	57.9	57.6	606-609 bp	1.0	78.1
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	GCCCCGGATCGTTAGATAAG	45.0	55.0	58.3	57.6	583-586 bp	1.0	76.4
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	GCCCCGGATCGTTAGATAAG	45.0	55.0	58.3	57.6	584-587 bp	1.0	76.4
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	GCCCCGGATCGTTAGATAAG	45.0	55.0	58.3	57.6	585-588 bp	1.0	76.4