Hotspot

trnK-UUU-rps16

LSC · 4435-4910 · 476 bp

Back to Aerides

Pi 0.00316

MarkerSeek score 61.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 476
variable_sites: 3
pi: 0.00316
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGGATCAGTCGCGGTCTTAT	GCTCAACCCACAGAAACTGT	50.0	50.0	58.3	58.3	665-702 bp	1.0	79.3
trnK-UUU-rps16_p2	AGGATCAGTCGCGGTCTTAT	GTGCTCAACCCACAGAAACT	50.0	50.0	58.3	58.3	667-704 bp	1.0	79.3
trnK-UUU-rps16_p3	AGGATCAGTCGCGGTCTTAT	TGCTCAACCCACAGAAACTG	50.0	50.0	58.3	58.3	666-703 bp	1.0	79.3
trnK-UUU-rps16_p4	GTCGCAATTAAAAGCCGAGT	GCTCAACCCACAGAAACTGT	45.0	50.0	57.1	58.3	590-627 bp	1.0	74.7
trnK-UUU-rps16_p5	GTCGCAATTAAAAGCCGAGT	GTGCTCAACCCACAGAAACT	45.0	50.0	57.1	58.3	592-629 bp	1.0	74.7