Hotspot

rps15

SSC · 120590-120868 · 279 bp

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Pi 0.00639

MarkerSeek score 29.70

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 279
variable_sites: 6
pi: 0.00639
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TCCTACTCCCTCAACACGTT	TTGAACCGGTTCGTATGTCC	50.0	50.0	58.0	57.9	1692 bp	1.0	80.7
rps15_p2	GTTATGGCCTCGGTGAACAT	TTGAACCGGTTCGTATGTCC	50.0	50.0	58.0	57.9	1316 bp	1.0	80.7
rps15_p3	TCCTACTCCCTCAACACGTT	ATTTCTGGCCCAGTTTTGGT	50.0	45.0	58.0	57.9	1378 bp	1.0	80.4
rps15_p4	GTTATGGCCTCGGTGAACAT	ATTTCTGGCCCAGTTTTGGT	50.0	45.0	58.0	57.9	1002 bp	1.0	80.4
rps15_p5	TCCTACTCCCTCAACACGTT	TTTCTGGCCCAGTTTTGGTT	50.0	45.0	58.0	58.1	1377 bp	1.0	80.2