Hotspot

psbA

LSC · 325-1386 · 1062 bp

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Pi 0.000171

MarkerSeek score 30.10

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTCATATAGACTAATACCGAAGT	GCTTGGGAGTCCCTGATGAT	33.3	55.0	54.2	59.2	1128 bp	1.0	40.1
psbA_p2	ACTCATATAGACTAATACCGAAGT	TTGCTTGGGAGTCCCTGAT	33.3	52.6	54.2	58.2	1130 bp	1.0	40.1
psbA_p3	ACTCATATAGACTAATACCGAAGT	CTTGGGAGTCCCTGATGATT	33.3	50.0	54.2	56.6	1127 bp	1.0	40.1
psbA_p4	AACTCATATAGACTAATACCGAAGT	GCTTGGGAGTCCCTGATGAT	32.0	55.0	54.9	59.2	1129 bp	1.0	40.1
psbA_p5	ACTCATATAGACTAATACCGAAGTT	GCTTGGGAGTCCCTGATGAT	32.0	55.0	54.9	59.2	1128 bp	1.0	40.1