Hotspot

ndhI

SSC · 116638-117141 · 504 bp

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Pi 0.00245

MarkerSeek score 28.70

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 504
variable_sites: 4
pi: 0.00245
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AAATAAGAAGTTGGGCCGCA	CTTTCACTCTAACCACGCGA	45.0	50.0	58.1	57.9	899-1058 bp	1.0	84.4
ndhI_p2	AAATAAGAAGTTGGGCCGCA	TTTCACTCTAACCACGCGAG	45.0	50.0	58.1	57.9	898-1057 bp	1.0	84.4
ndhI_p3	AAATAAGAAGTTGGGCCGCA	TTACTTGGGCGGTTGGAATC	45.0	50.0	58.1	58.2	1152-1311 bp	1.0	83.3
ndhI_p4	TCGTTCAATTCGGCAGTTCA	CTTTCACTCTAACCACGCGA	45.0	50.0	58.1	57.9	675-692 bp	0.909091	77.0
ndhI_p5	TCGTTCAATTCGGCAGTTCA	TTTCACTCTAACCACGCGAG	45.0	50.0	58.1	57.9	674-691 bp	0.909091	77.0