Hotspot

ndhG-ndhI

SSC · 116380-116637 · 258 bp

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Pi 0.0168

MarkerSeek score 60.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 258
variable_sites: 16
pi: 0.0168
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AATCATGTATTGGTCCCGGT	AATCAAATTGCGTTGGGTCG	45.0	45.0	56.6	57.7	231-388 bp	1.0	73.4
ndhG-ndhI_p2	ATTGGTCCCGGTAAATCCAT	AATCAAATTGCGTTGGGTCG	45.0	45.0	56.6	57.7	223-380 bp	1.0	73.2
ndhG-ndhI_p3	GTATTGGTCCCGGTAAATCCA	AATCAAATTGCGTTGGGTCG	47.6	45.0	57.4	57.7	225-382 bp	1.0	72.2
ndhG-ndhI_p4	TGTATTGGTCCCGGTAAATCC	AATCAAATTGCGTTGGGTCG	47.6	45.0	57.4	57.7	226-383 bp	1.0	72.2
ndhG-ndhI_p5	AAATCATGTATTGGTCCCGGT	AATCAAATTGCGTTGGGTCG	42.9	45.0	57.3	57.7	232-389 bp	1.0	71.4