Hotspot

ndhF-rpl32

SSC · 110620-111195 · 576 bp

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Pi 0.00941
MarkerSeek score 55.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
576
variable_sites
17
pi
0.00941
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTTTGTTGCCGTCGGAAAAA CGCTACCCAATATCCCTTCC 40.0 55.0 57.4 57.5 743-762 bp 1.0 74.9
ndhF-rpl32_p2 TTTTTGTTGCCGTCGGAAAA CGCTACCCAATATCCCTTCC 40.0 55.0 57.4 57.5 744-763 bp 1.0 74.9
ndhF-rpl32_p3 TTTTGTTGCCGTCGGAAAAA AAGGCTTTTAACGCTACCCA 40.0 45.0 57.4 57.4 754-773 bp 1.0 74.0
ndhF-rpl32_p4 TTTTGTTGCCGTCGGAAAAA AGGCTTTTAACGCTACCCAA 40.0 45.0 57.4 57.4 753-772 bp 1.0 74.0
ndhF-rpl32_p5 TTTTTGTTGCCGTCGGAAAA AGGCTTTTAACGCTACCCAA 40.0 45.0 57.4 57.4 754-773 bp 1.0 74.0