Hotspot

psaJ-rpl33

LSC · 65592-65984 · 393 bp

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Pi 0.00689

MarkerSeek score 54.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 393
variable_sites: 8
pi: 0.00689
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGTTCGCATCTTTAGCAGGT	ACATTGGTTCGAACACAACTG	50.0	42.9	58.3	57.3	541-547 bp	1.0	66.3
psaJ-rpl33_p2	TGGTTCGCATCTTTAGCAGG	ACATTGGTTCGAACACAACTG	50.0	42.9	58.3	57.3	542-548 bp	1.0	66.3
psaJ-rpl33_p3	GCGCTGACATTTCCCTTTTT	ACATTGGTTCGAACACAACTG	45.0	42.9	57.6	57.3	488-494 bp	1.0	65.1
psaJ-rpl33_p4	ATGCGCTGACATTTCCCTTT	ACATTGGTTCGAACACAACTG	45.0	42.9	58.5	57.3	490-496 bp	1.0	65.0
psaJ-rpl33_p5	ATCGTTTATTCCCGGATGCG	ACATTGGTTCGAACACAACTG	50.0	42.9	58.5	57.3	505-511 bp	1.0	64.6