Hotspot

trnE-UUC-trnT-GGU

LSC · 31086-31838 · 753 bp

Back to Aeonium

Pi 0.0041
MarkerSeek score 53.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
753
variable_sites
13
pi
0.0041
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CATAGTACCCTACCCCCAGG GAACCGATGACTTACGCCTT 60.0 50.0 58.0 58.0 864-898 bp 1.0 83.4
trnE-UUC-trnT-GGU_p2 CATAGTACCCTACCCCCAGG CCATGGCGTTACTCTACCAC 60.0 55.0 58.0 58.1 843-877 bp 1.0 83.1
trnE-UUC-trnT-GGU_p3 CATAGTACCCTACCCCCAGG TGGCGTTACTCTACCACTGA 60.0 50.0 58.0 58.1 840-874 bp 1.0 82.9
trnE-UUC-trnT-GGU_p4 CATAGTACCCTACCCCCAGG CCGATGACTTACGCCTTACC 60.0 55.0 58.0 58.2 861-895 bp 1.0 82.3
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 829-863 bp 1.0 81.7