Hotspot

trnG-GCC-trnR-UCU

LSC · 9829-10029 · 201 bp

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Pi 0.0167

MarkerSeek score 62.60

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 201
variable_sites: 9
pi: 0.0167
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	TAGCCTTCCAAGCTACCGAT	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	58.2	55.6	281-303 bp	1.0	49.8
trnG-GCC-trnR-UCU_p2	TCCTGAGTTGACGTCGACTA	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	57.8	55.6	308-330 bp	1.0	49.7
trnG-GCC-trnR-UCU_p3	TCCTGAGTTGACGTCGACTA	AGAAGACCTATGTCCTATCCA	50.0	42.9	57.8	54.6	302-324 bp	1.0	49.6
trnG-GCC-trnR-UCU_p4	TAGCCTTCCAAGCTACCGAT	AGAAGACCTATGTCCTATCCA	50.0	42.9	58.2	54.6	275-297 bp	1.0	49.6
trnG-GCC-trnR-UCU_p5	TCCTGAGTTGACGTCGACTA	ACCAAAGGTTTAGAAGACCTATGT	50.0	37.5	57.8	57.6	313-335 bp	1.0	49.0