Hotspot

trnH-GUG-psbA

LSC · 73-324 · 252 bp

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Pi 0.0229

MarkerSeek score 64.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 252
variable_sites: 17
pi: 0.0229
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GTAACGCTCACAACTTCCCT	55.0	50.0	58.1	57.8	329-344 bp	1.0	83.4
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GTAACGCTCACAACTTCCCT	50.0	50.0	57.9	57.8	343-358 bp	1.0	83.1
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GTAACGCTCACAACTTCCCT	50.0	50.0	57.9	57.8	342-357 bp	1.0	83.1
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GTAACGCTCACAACTTCCCT	50.0	50.0	57.9	57.8	344-359 bp	1.0	83.1
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TGAACGTAACGCTCACAACT	55.0	45.0	58.1	57.8	334-349 bp	1.0	82.9