Hotspot

rps2

LSC · 30221-30931 · 711 bp

Back to Aeluropus

Pi 0.00375

MarkerSeek score 61.10

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 4
pi: 0.00375
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TCAATTCCGTAATGGTAAGACGA	TCCCATAAATCTACGTAGTTGCT	39.1	39.1	57.7	57.1	817-818 bp	1.0	40.4
rps2_p2	TCAATTCCGTAATGGTAAGACGA	TCCCATAAATCTACGTAGTTGCTT	39.1	37.5	57.7	57.8	817-818 bp	1.0	40.4
rps2_p3	CAATTCCGTAATGGTAAGACGA	TCCCATAAATCTACGTAGTTGCT	40.9	39.1	56.3	57.1	816-817 bp	1.0	40.4
rps2_p4	TCAATTCCGTAATGGTAAGACG	TCCCATAAATCTACGTAGTTGCT	40.9	39.1	56.3	57.1	817-818 bp	1.0	40.4
rps2_p5	TCAATTCCGTAATGGTAAGACGA	TCCCATAAATCTACGTAGTTGC	39.1	40.9	57.7	55.7	817-818 bp	1.0	40.4