Hotspot

ndhA

SSC · 111190-113329 · 2140 bp

Back to Aeluropus

Pi 0.00561

MarkerSeek score 47.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2140
variable_sites: 18
pi: 0.00561
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ATTGGGCATACACGAACACA	GGCTTATCCGGACCAATGTT	45.0	50.0	57.8	57.9	2992 bp	1.0	78.7
ndhA_p2	TTGGGCATACACGAACACAT	GGCTTATCCGGACCAATGTT	45.0	50.0	57.8	57.9	2991 bp	1.0	78.7
ndhA_p3	TTGGGCATACACGAACACAT	AAATTGGGGCTTATCCGGAC	45.0	50.0	57.8	57.9	2998 bp	1.0	78.6
ndhA_p4	CTGGTAACCGACTCAAAGCA	GCTCGACGCTTTAAGAATGC	50.0	50.0	57.8	57.9	2982 bp	0.333333	51.4
ndhA_p5	ACTGGTAACCGACTCAAAGC	GCTCGACGCTTTAAGAATGC	50.0	50.0	57.8	57.9	2983 bp	0.333333	51.4