Hotspot

ndhF-rpl32

SSC · 104021-104929 · 909 bp

Back to Aeluropus

Pi 0.0066

MarkerSeek score 51.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 909
variable_sites: 9
pi: 0.0066
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACCCAGGCATATTGATATGT	TGACATCGAAGTACGTTTTT	40.0	35.0	53.8	53.2	971-976 bp	1.0	41.4
ndhF-rpl32_p2	ACCCAGGCATATTGATATGT	TTGACATCGAAGTACGTTTT	40.0	35.0	53.8	53.2	972-977 bp	1.0	41.4
ndhF-rpl32_p3	ACCCAGGCATATTGATATGT	TTTGACATCGAAGTACGTTT	40.0	35.0	53.8	53.2	973-978 bp	1.0	41.4
ndhF-rpl32_p4	ACCCAGGCATATTGATATGT	TTTTGACATCGAAGTACGTT	40.0	35.0	53.8	53.2	974-979 bp	1.0	41.4
ndhF-rpl32_p5	ACCCAGGCATATTGATATGT	TTTTTGACATCGAAGTACGT	40.0	35.0	53.8	53.2	975-980 bp	1.0	41.4