Hotspot

rps3-rpl22

LSC · 80253-80314 · 62 bp

Back to Aeluropus

Pi 0.0109

MarkerSeek score 64.60

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 62
variable_sites: 1
pi: 0.0109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	GTGCGAACCAAAAGGAATGG	AAAATTCAGACCTCGGGCTC	50.0	50.0	57.9	57.9	228-232 bp	1.0	80.3
rps3-rpl22_p2	ACCAAGTCTGAAACCGAGTG	AAAATTCAGACCTCGGGCTC	50.0	50.0	57.8	57.9	194-198 bp	1.0	79.2
rps3-rpl22_p3	CCAAGTCTGAAACCGAGTGG	AAAATTCAGACCTCGGGCTC	55.0	50.0	58.5	57.9	193-197 bp	1.0	77.2
rps3-rpl22_p4	CAAGTCTGAAACCGAGTGGA	AAAATTCAGACCTCGGGCTC	50.0	50.0	57.5	57.9	192-196 bp	1.0	76.9
rps3-rpl22_p5	GTGCGAACCAAAAGGAATGG	AAAAATTCAGACCTCGGGCT	50.0	45.0	57.9	57.4	229-233 bp	1.0	76.5