Hotspot

trnP-UGG-psaJ

LSC · 64563-64948 · 386 bp

Back to Aeluropus

Pi 0.0104

MarkerSeek score 51.00

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 6
pi: 0.0104
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	ACGGGTGCTACAGAGAGATA	45.0	50.0	57.9	57.3	460 bp	1.0	74.3
trnP-UGG-psaJ_p2	CAAAACAAACGCGCTACCAA	ACGGGTGCTACAGAGAGATA	45.0	50.0	58.2	57.3	461 bp	1.0	73.8
trnP-UGG-psaJ_p3	TTGTACCCAAAACAAACGCG	ACGGGTGCTACAGAGAGATA	45.0	50.0	57.8	57.3	468 bp	1.0	73.7
trnP-UGG-psaJ_p4	AAAACAAACGCGCTACCAAG	CACGGGTGCTACAGAGAGAT	45.0	55.0	57.9	58.9	461 bp	1.0	73.0
trnP-UGG-psaJ_p5	CAAAACAAACGCGCTACCAA	CACGGGTGCTACAGAGAGAT	45.0	55.0	58.2	58.9	462 bp	1.0	72.5