Hotspot

ndhC-trnV-UAC

LSC · 50620-51540 · 921 bp

Back to Aeluropus

Pi 0.00869
MarkerSeek score 64.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
921
variable_sites
12
pi
0.00869
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGTCCTTCATTAATCGGGGC GGTTCGAATCCGTATAGCCC 55.0 55.0 58.4 57.9 1056-1057 bp 1.0 77.1
ndhC-trnV-UAC_p2 GGTCCTTCATTAATCGGGGC AGCCCAATGCAGAAAAATGC 55.0 45.0 58.4 57.9 1149-1150 bp 1.0 76.9
ndhC-trnV-UAC_p3 GGTCCTTCATTAATCGGGGC CGGTTCGAATCCGTATAGCC 55.0 55.0 58.4 58.3 1057-1058 bp 1.0 75.3
ndhC-trnV-UAC_p4 GTCCTTCATTAATCGGGGCT GGTTCGAATCCGTATAGCCC 50.0 55.0 57.4 57.9 1055-1056 bp 1.0 75.2
ndhC-trnV-UAC_p5 GTCCTTCATTAATCGGGGCT AGCCCAATGCAGAAAAATGC 50.0 45.0 57.4 57.9 1148-1149 bp 1.0 74.9