Hotspot

psbM-petN

LSC · 17206-17800 · 595 bp

Back to Aeluropus

Pi 0.0045

MarkerSeek score 64.00

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 595
variable_sites: 4
pi: 0.0045
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACAGCCAGCCAAAATGA	AGTATGGGGGAGAAGTGGAC	40.0	55.0	56.7	58.1	628-644 bp	1.0	71.2
psbM-petN_p2	AAAACAGCCAGCCAAAATGA	GTATGGGGGAGAAGTGGACT	40.0	55.0	56.7	58.1	627-643 bp	1.0	71.2
psbM-petN_p3	AAAACAGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.7	58.2	686-702 bp	1.0	70.7
psbM-petN_p4	AAAACAGCCAGCCAAAATGA	TATGGGGGAGAAGTGGACTC	40.0	55.0	56.7	57.8	626-642 bp	1.0	70.7
psbM-petN_p5	AAAACAGCCAGCCAAAATGA	GGGGGAGAAGTGGACTCTAG	40.0	60.0	56.7	58.2	623-639 bp	1.0	70.4