Hotspot

trnY-GUA-trnD-GUC

LSC · 15613-15953 · 341 bp

Back to Aeluropus

Pi 0.00782

MarkerSeek score 51.00

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 341
variable_sites: 4
pi: 0.00782
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	AATTCTTCCTGGGTCGATGC	GCGGTGCTCTAACCAATTGA	50.0	50.0	58.0	58.6	466 bp	1.0	75.9
trnY-GUA-trnD-GUC_p2	GGGGACGGACTGTAAATTCG	GCGGTGCTCTAACCAATTGA	55.0	50.0	58.4	58.6	433 bp	1.0	73.5
trnY-GUA-trnD-GUC_p3	GGGACGGACTGTAAATTCGT	GCGGTGCTCTAACCAATTGA	50.0	50.0	57.6	58.6	432 bp	1.0	73.3
trnY-GUA-trnD-GUC_p4	AATTCTTCCTGGGTCGATGC	GGCGGTGCTCTAACCAATT	50.0	52.6	58.0	58.1	467 bp	1.0	71.3
trnY-GUA-trnD-GUC_p5	TGGGGACGGACTGTAAATTC	GCGGTGCTCTAACCAATTGA	50.0	50.0	57.2	58.6	434 bp	1.0	70.1