Hotspot

trnT-GGU-trnE-UUC

LSC · 14920-15394 · 475 bp

Back to Aeluropus

Pi 0.00982

MarkerSeek score 62.40

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 475
variable_sites: 7
pi: 0.00982
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GTGGTAGAGTAATGCCACGG	GCTGCCTCCTTGAAAGAGAG	55.0	55.0	58.1	58.3	577-584 bp	1.0	78.3
trnT-GGU-trnE-UUC_p2	GTGGTAGAGTAATGCCACGG	TGTCCTGAACCACTAGACGA	55.0	50.0	58.1	57.7	556-563 bp	1.0	78.2
trnT-GGU-trnE-UUC_p3	GTGGTAGAGTAATGCCACGG	AACCACTAGACGATAGGGGC	55.0	55.0	58.1	58.6	549-556 bp	1.0	75.8
trnT-GGU-trnE-UUC_p4	GTGGTAGAGTAATGCCACGG	TAATACTCCCTACCCCCAGG	55.0	55.0	58.1	56.9	612-619 bp	1.0	71.5
trnT-GGU-trnE-UUC_p5	CGGTAGAGTAATGCCATGGT	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	57.1	58.3	643-650 bp	1.0	71.5