Hotspot

psbI-trnS-GCU

LSC · 7953-8071 · 119 bp

Back to Aeluropus

Pi 0.028

MarkerSeek score 66.60

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 119
variable_sites: 5
pi: 0.028
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	AATCCTGGACGTGAGGAGTA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.5	58.0	208 bp	1.0	77.4
psbI-trnS-GCU_p2	ATCCTGGACGTGAGGAGTAA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.5	58.0	207 bp	1.0	77.4
psbI-trnS-GCU_p3	TAATCCTGGACGTGAGGAGT	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.5	58.0	209 bp	1.0	77.4
psbI-trnS-GCU_p4	ATCCTGGACGTGAGGAGTAA	CTTTCGATTTGGAACGGGGA	50.0	50.0	57.5	58.2	244 bp	1.0	76.0
psbI-trnS-GCU_p5	ATCCTGGACGTGAGGAGTAA	TTTCGATTTGGAACGGGGAG	50.0	50.0	57.5	58.2	243 bp	1.0	76.0