Hotspot

atpB

Genome · 15666-17065 · 1400 bp

Back to Aeginetia

Pi 0.0892

MarkerSeek score 78.50

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: Genome
length_bp: 1400
variable_sites: 108
pi: 0.0892
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	CGCTGTAGCAATAGGAGCAT	CGACCTAATTGTACCACGCA	50.0	50.0	57.8	58.0	1728 bp	0.5	58.7
atpB_p2	CGCTGTAGCAATAGGAGCAT	ACTGAGGCAGTTCAAACAGG	50.0	50.0	57.8	58.0	2189 bp	0.5	58.5
atpB_p3	CGCTGTAGCAATAGGAGCAT	CTGAAGGTTCACAAGTGGCT	50.0	50.0	57.8	58.0	1774 bp	0.5	58.5
atpB_p4	CGCTGTAGCAATAGGAGCAT	GAAGGTTCACAAGTGGCTGA	50.0	50.0	57.8	58.0	1772 bp	0.5	58.5
atpB_p5	CGCTGTAGCAATAGGAGCAT	TTCCGGTAGCAATTGGAGTG	50.0	50.0	57.8	57.9	2149 bp	0.5	57.8