Hotspot

atpA

Genome · 4598-5941 · 1344 bp

Back to Aeginetia

Pi 0.112

MarkerSeek score 80.80

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: Genome
length_bp: 1344
variable_sites: 147
pi: 0.112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	ACGGAAAGAGAGGGATTCGA	AGTTGTTTTACCGCCGAGTT	50.0	45.0	58.2	58.0	1966 bp	0.5	58.5
atpA_p2	GTTGCCTTACCACTTAGCCA	AGTTGTTTTACCGCCGAGTT	50.0	45.0	57.8	58.0	2158 bp	0.5	58.1
atpA_p3	TTGCCTTACCACTTAGCCAC	AGTTGTTTTACCGCCGAGTT	50.0	45.0	57.8	58.0	2157 bp	0.5	58.1
atpA_p4	CCCGTTGCCTTACCACTTAG	AGTTGTTTTACCGCCGAGTT	55.0	45.0	58.3	58.0	2161 bp	0.5	57.5
atpA_p5	AAAAGATATGCCCTGGGACG	AGTTGTTTTACCGCCGAGTT	50.0	45.0	57.7	58.0	2215 bp	0.5	56.9