Hotspot

trnS-GGA-rps4

Genome · 12330-12705 · 376 bp

Back to Aeginetia

Pi 0.156

MarkerSeek score 81.90

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: Genome
length_bp: 376
variable_sites: 53
pi: 0.156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	ATGTAGGAGAGATGGCCGAG	AAATGAATTGCTAGTCGTAGA	55.0	33.3	58.4	52.7	493-510 bp	1.0	52.0
trnS-GGA-rps4_p2	ATGTAGGAGAGATGGCCGAG	AATGAATTGCTAGTCGTAGAA	55.0	33.3	58.4	52.7	492-509 bp	1.0	52.0
trnS-GGA-rps4_p3	ATGTAGGAGAGATGGCCGAG	TGCTAGTCGTAGAATATTATTCTCGT	55.0	34.6	58.4	57.7	485-502 bp	1.0	52.0
trnS-GGA-rps4_p4	ATGTAGGAGAGATGGCCGAG	AAATGAATTGCTAGTCGTAGAA	55.0	31.8	58.4	53.6	493-510 bp	1.0	52.0
trnS-GGA-rps4_p5	ATGTAGGAGAGATGGCCGAG	ATGAATTGCTAGTCGTAGAAT	55.0	33.3	58.4	52.5	491-508 bp	1.0	52.0