Hotspot

trnY-GUA-trnE-UUC

Genome · 9468-9530 · 63 bp

Back to Aeginetia

Pi 0.127

MarkerSeek score 78.50

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: Genome
length_bp: 63
variable_sites: 8
pi: 0.127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	ACGAATTTACAGTCCGTCCC	CAAGGAGACAGCGAGGATTC	50.0	55.0	57.6	58.1	141-156 bp	1.0	80.8
trnY-GUA-trnE-UUC_p2	CAGTCCGTCCCTATTAACCG	CAAGGAGACAGCGAGGATTC	55.0	55.0	57.5	58.1	132-147 bp	1.0	80.0
trnY-GUA-trnE-UUC_p3	CTATTAACCGCTCGGGCATC	CAAGGAGACAGCGAGGATTC	55.0	55.0	58.6	58.1	122-137 bp	1.0	79.8
trnY-GUA-trnE-UUC_p4	ACGAATTTACAGTCCGTCCC	GAGGATTCGAATTCCCCTGG	50.0	55.0	57.6	57.7	129-144 bp	1.0	79.6
trnY-GUA-trnE-UUC_p5	GCCAACGAATTTACAGTCCG	CAAGGAGACAGCGAGGATTC	50.0	55.0	57.5	58.1	145-160 bp	1.0	79.5