Hotspot

psaC-ndhE

SSC · 109801-110277 · 477 bp

Back to Aegilops

Pi 0.00462

MarkerSeek score 54.70

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 477
variable_sites: 8
pi: 0.00462
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GCTTTACATCCATCCCAGGG	AGCCGCTATTGGACTATCCA	55.0	50.0	58.0	58.3	656-692 bp	1.0	81.4
psaC-ndhE_p2	GGCTTTACATCCATCCCAGG	AGCCGCTATTGGACTATCCA	55.0	50.0	58.0	58.3	657-693 bp	1.0	81.4
psaC-ndhE_p3	GTTGGACAAGCTCGTACACA	AGCCGCTATTGGACTATCCA	50.0	50.0	58.1	58.3	617-653 bp	1.0	80.7
psaC-ndhE_p4	TGTTGGACAAGCTCGTACAC	AGCCGCTATTGGACTATCCA	50.0	50.0	58.1	58.3	618-654 bp	1.0	80.7
psaC-ndhE_p5	CTGTTGGACAAGCTCGTACA	AGCCGCTATTGGACTATCCA	50.0	50.0	57.9	58.3	619-655 bp	1.0	80.6