Hotspot

ndhF-rpl32

SSC · 104833-105740 · 908 bp

Back to Aegilops

Pi 0.00601
MarkerSeek score 40.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
908
variable_sites
14
pi
0.00601
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TTGACATTGAAGTACGTTTTT 55.0 28.6 58.1 52.2 980-1024 bp 1.0 42.1
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TTTGACATTGAAGTACGTTTT 55.0 28.6 58.1 52.2 981-1025 bp 1.0 42.1
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTTTGACATTGAAGTACGTTT 55.0 28.6 58.1 52.2 982-1026 bp 1.0 42.1
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTTTGACATTGAAGTACGTT 55.0 28.6 58.1 52.2 983-1027 bp 1.0 42.1
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TCCAAATATTTTTACGAATACGCT 55.0 29.2 58.1 55.3 1007-1051 bp 1.0 42.0