Hotspot

infA-rps8

LSC · 76943-77115 · 173 bp

Back to Aegilops

Pi 0.008

MarkerSeek score 52.40

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 173
variable_sites: 5
pi: 0.008
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	TGGTGTCATTCTCTAGCCGA	GGGTGGAATGGGAATTGCTA	50.0	50.0	58.2	57.5	308-325 bp	1.0	78.0
infA-rps8_p2	AGCCTAGGATGATGGTGTCA	GGGTGGAATGGGAATTGCTA	50.0	50.0	57.8	57.5	320-337 bp	1.0	77.7
infA-rps8_p3	TTGACCCTATCCCCCATCAG	GGGTGGAATGGGAATTGCTA	55.0	50.0	58.2	57.5	378-395 bp	1.0	77.2
infA-rps8_p4	TTTGACCCTATCCCCCATCA	GGGTGGAATGGGAATTGCTA	50.0	50.0	57.7	57.5	379-396 bp	1.0	76.3
infA-rps8_p5	TGGTGTCATTCTCTAGCCGA	TTGGGTGGAATGGGAATTGC	50.0	50.0	58.2	58.7	310-327 bp	1.0	75.8