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petG-LK95_t035

LSC · 64361-64481 · 121 bp

Back to Aegilops

Pi 0.042

MarkerSeek score 63.90

Primer available yes

Label petG-LK95_t035

Feature Metadata

label_name: petG-LK95_t035
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 10
pi: 0.042
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-LK95_t035_p1	GGGATTATTCGTGACTGCGT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	253-254 bp	1.0	82.8
petG-LK95_t035_p2	GGGATTATTCGTGACTGCGT	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	217-218 bp	1.0	82.8
petG-LK95_t035_p3	AGCGGGATTATTCGTGACTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	256-257 bp	1.0	81.5
petG-LK95_t035_p4	GCGTATTTGCAATACAGGCG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.3	58.0	237-238 bp	1.0	81.5
petG-LK95_t035_p5	AGCGGGATTATTCGTGACTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	220-221 bp	1.0	81.5