Hotspot

LK95_t020-psbM

LSC · 15819-16633 · 815 bp

Back to Aegilops

Pi 0.00456
MarkerSeek score 49.70
Primer available yes
Label LK95_t020-psbM

Feature Metadata

label_name
LK95_t020-psbM
feature_type
igs
region
LSC
length_bp
815
variable_sites
9
pi
0.00456
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
LK95_t020-psbM_p1 TTCAATTGGTCAGAGCACCG AGAATGAACAGTGCAGTAGC 50.0 45.0 58.5 55.8 923-926 bp 1.0 59.6
LK95_t020-psbM_p2 GTTCAATTGGTCAGAGCACC AGAATGAACAGTGCAGTAGC 50.0 45.0 57.0 55.8 924-927 bp 1.0 55.4
LK95_t020-psbM_p3 CGCGGGGATTGTAGTTCAA AGAATGAACAGTGCAGTAGC 52.6 45.0 57.9 55.8 937-940 bp 1.0 54.3
LK95_t020-psbM_p4 TTCAATTGGTCAGAGCACCG AGGAACTAGAATGAACAGTGCA 50.0 40.9 58.5 57.4 930-933 bp 0.923077 53.2
LK95_t020-psbM_p5 TTCAATTGGTCAGAGCACCG GGAACTAGAATGAACAGTGCAG 50.0 45.5 58.5 57.1 929-932 bp 0.923077 50.7