Hotspot

LK95_t012-LK95_t014

LSC · 12761-14725 · 1965 bp

Back to Aegilops

Pi 0.0026

MarkerSeek score 51.30

Primer available yes

Label LK95_t012-LK95_t014

Feature Metadata

label_name: LK95_t012-LK95_t014
feature_type: igs
region: LSC
length_bp: 1965
variable_sites: 15
pi: 0.0026
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
LK95_t012-LK95_t014_p1	CCTCAAGGTTATGAGCCTCG	CGACTTACGTCTTAGCACGA	55.0	50.0	57.8	57.5	1578-2157 bp	1.0	78.6
LK95_t012-LK95_t014_p2	CCTCGTGAGCTACCAAACTG	CGACTTACGTCTTAGCACGA	55.0	50.0	58.3	57.5	1563-2142 bp	1.0	78.2
LK95_t012-LK95_t014_p3	CAAGGTTATGAGCCTCGTGA	CGACTTACGTCTTAGCACGA	50.0	50.0	57.3	57.5	1575-2154 bp	1.0	75.1
LK95_t012-LK95_t014_p4	TCAAGGTTATGAGCCTCGTG	CGACTTACGTCTTAGCACGA	50.0	50.0	57.3	57.5	1576-2155 bp	1.0	75.1
LK95_t012-LK95_t014_p5	TATGAGCCTCGTGAGCTACC	CGACTTACGTCTTAGCACGA	55.0	50.0	58.7	57.5	1569-2148 bp	1.0	75.0