Hotspot

LK95_t006-psbD

LSC · 7626-8663 · 1038 bp

Back to Aegilops

Pi 0.00595

MarkerSeek score 54.00

Primer available yes

Label LK95_t006-psbD

Feature Metadata

label_name: LK95_t006-psbD
feature_type: igs
region: LSC
length_bp: 1038
variable_sites: 15
pi: 0.00595
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
LK95_t006-psbD_p1	ATTAGCAATCCGCCGCTTTA	GAGCAATAGGCCAGACCATC	45.0	55.0	58.0	58.1	1175-1211 bp	1.0	81.6
LK95_t006-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1153-1189 bp	1.0	80.6
LK95_t006-psbD_p3	ATTAGCAATCCGCCGCTTTA	CCCTTCGTAACCAGTCATCC	45.0	55.0	58.0	57.7	1137-1173 bp	1.0	80.1
LK95_t006-psbD_p4	GAGCGGAAAGAGAGGGATTC	GAGCAATAGGCCAGACCATC	55.0	55.0	57.8	58.1	1226-1262 bp	1.0	80.0
LK95_t006-psbD_p5	ATTAGCAATCCGCCGCTTTA	AGGCCAGACCATCCTACAAA	45.0	50.0	58.0	58.3	1168-1204 bp	1.0	79.9