Hotspot

psbK-psbI

LSC · 6914-7281 · 368 bp

Back to Aegilops

Pi 0.0185

MarkerSeek score 57.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 368
variable_sites: 20
pi: 0.0185
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	TGGCAAGCTGCTGTAAGTTT	ACAAAAAGTTTGAGAGTAAGCA	45.0	31.8	58.3	54.4	444-455 bp	1.0	45.7
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	ACAAAAAGTTTGAGAGTAAGCA	50.0	31.8	57.9	54.4	447-458 bp	1.0	45.6
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	AACAAAAAGTTTGAGAGTAAGCA	50.0	30.4	57.9	55.2	448-459 bp	1.0	45.6
psbK-psbI_p4	TGCCAAATTACCCGAAGCTT	ACAAAAAGTTTGAGAGTAAGCA	45.0	31.8	58.1	54.4	535-546 bp	1.0	44.7
psbK-psbI_p5	TTGCCAAATTACCCGAAGCT	ACAAAAAGTTTGAGAGTAAGCA	45.0	31.8	58.1	54.4	536-547 bp	1.0	44.7