Hotspot

tRNAK-rps16

LSC · 3945-4513 · 569 bp

Back to Aegilops

Pi 0.0107

MarkerSeek score 39.50

Primer available yes

Label tRNAK-rps16

Feature Metadata

label_name: tRNAK-rps16
feature_type: igs
region: LSC
length_bp: 569
variable_sites: 16
pi: 0.0107
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNAK-rps16_p1	TGAGGATCAGTCGTGGTCTT	TCGAGCCGTATGAGGAGAAA	50.0	50.0	58.1	58.2	991-993 bp	1.0	79.3
tRNAK-rps16_p2	TGAGGATCAGTCGTGGTCTT	TTTCTCGAGCCGTATGAGGA	50.0	50.0	58.1	58.2	995-997 bp	1.0	79.3
tRNAK-rps16_p3	ACGTCTAATTAGAGCGGGGA	TCGAGCCGTATGAGGAGAAA	50.0	50.0	57.9	58.2	1172-1174 bp	1.0	79.2
tRNAK-rps16_p4	ACGTCTAATTAGAGCGGGGA	TTTCTCGAGCCGTATGAGGA	50.0	50.0	57.9	58.2	1176-1178 bp	1.0	79.2
tRNAK-rps16_p5	TAGGAAGTTCAGCTCGTTGC	TCGAGCCGTATGAGGAGAAA	50.0	50.0	57.9	58.2	1141-1143 bp	1.0	79.1