Hotspot

rps16

LSC · 4912-5975 · 1064 bp

Back to Adromischus

Pi 0.00878

MarkerSeek score 55.50

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1064
variable_sites: 18
pi: 0.00878
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ATCCGAGTCATACCCACACT	TGACTACTCATGATTCCATTTCT	50.0	34.8	57.8	55.1	1116-1129 bp	1.0	43.1
rps16_p2	AGATCCGAGTCATACCCACA	TGACTACTCATGATTCCATTTCT	50.0	34.8	57.5	55.1	1118-1131 bp	1.0	43.1
rps16_p3	ATCCGAGTCATACCCACACT	ACCAAACTAATGACTACTCATGA	50.0	34.8	57.8	55.1	1126-1139 bp	1.0	43.0
rps16_p4	ATCCGAGTCATACCCACACT	CCAAACTAATGACTACTCATGATTCC	50.0	38.5	57.8	57.9	1125-1138 bp	1.0	43.0
rps16_p5	AGATCCGAGTCATACCCACA	ACCAAACTAATGACTACTCATGA	50.0	34.8	57.5	55.1	1128-1141 bp	1.0	43.0