Hotspot

trnP-UGG-psaJ

LSC · 65531-65879 · 349 bp

Back to Adromischus

Pi 0.01

MarkerSeek score 62.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 349
variable_sites: 7
pi: 0.01
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	TACTTAGTACCGGTGCCACA	45.0	50.0	57.9	58.1	424-437 bp	1.0	81.4
trnP-UGG-psaJ_p2	AAAACAAACGCGCTACCAAG	GTCAGCGCATCTGGGAATAA	45.0	50.0	57.9	58.0	491-504 bp	1.0	81.3
trnP-UGG-psaJ_p3	CAAAACAAACGCGCTACCAA	TACTTAGTACCGGTGCCACA	45.0	50.0	58.2	58.1	425-438 bp	1.0	81.0
trnP-UGG-psaJ_p4	CAAAACAAACGCGCTACCAA	GTCAGCGCATCTGGGAATAA	45.0	50.0	58.2	58.0	492-505 bp	1.0	80.8
trnP-UGG-psaJ_p5	TTGTACCCAAAACAAACGCG	GTCAGCGCATCTGGGAATAA	45.0	50.0	57.8	58.0	499-512 bp	1.0	80.6