Hotspot

psbM-trnD-GUC

LSC · 29909-30878 · 970 bp

Back to Adromischus

Pi 0.0112

MarkerSeek score 59.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 970
variable_sites: 21
pi: 0.0112
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TCAATTGGTAAGAGCACCGC	45.0	50.0	55.8	58.6	1080-1112 bp	1.0	60.2
psbM-trnD-GUC_p2	AGGAACTAGAATGAACAGTGCA	TCAATTGGTAAGAGCACCGC	40.9	50.0	57.4	58.6	1087-1119 bp	1.0	57.1
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	AATTGGTAAGAGCACCGCC	45.0	52.6	55.8	58.1	1078-1110 bp	1.0	55.6
psbM-trnD-GUC_p4	GGAACTAGAATGAACAGTGCAG	TCAATTGGTAAGAGCACCGC	45.5	50.0	57.1	58.6	1086-1118 bp	1.0	54.6
psbM-trnD-GUC_p5	GGCAGTAGGAACTAGAATGAACA	TCAATTGGTAAGAGCACCGC	43.5	50.0	57.9	58.6	1093-1125 bp	1.0	53.4