Hotspot

rps16-trnQ-UUG

LSC · 5976-7472 · 1497 bp

Back to Adromischus

Pi 0.0185
MarkerSeek score 63.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1497
variable_sites
45
pi
0.0185
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1130-1576 bp 1.0 84.8
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1141-1587 bp 1.0 84.3
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTATGCGGAGGTTCGAATCC 45.0 55.0 58.1 58.2 1137-1583 bp 1.0 83.7
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC CTATGCGGAGGTTCGAATCC 50.0 55.0 58.2 58.2 1148-1594 bp 1.0 83.2
rps16-trnQ-UUG_p5 TTCCACAGCTGATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 1153-1599 bp 1.0 82.6