Hotspot

psbA-trnK-UUU

LSC · 1445-1686 · 242 bp

Back to Adromischus

Pi 0.0172

MarkerSeek score 63.30

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 242
variable_sites: 8
pi: 0.0172
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	ATAGGCTTTCAGTTTCGCGT	GGCTTGGGGAGAGTCTTTTT	45.0	50.0	57.9	57.7	375-388 bp	1.0	78.6
psbA-trnK-UUU_p2	AAACGACCCCATAGGCTTTC	GGCTTGGGGAGAGTCTTTTT	50.0	50.0	57.9	57.7	385-398 bp	1.0	78.3
psbA-trnK-UUU_p3	CAAAAACGACCCCATAGGCT	GGCTTGGGGAGAGTCTTTTT	50.0	50.0	58.2	57.7	388-401 bp	1.0	78.0
psbA-trnK-UUU_p4	ATAGGCTTTCAGTTTCGCGT	AAAATGCAAACACGGCTTGG	45.0	45.0	57.9	58.4	388-401 bp	1.0	77.5
psbA-trnK-UUU_p5	AAACGACCCCATAGGCTTTC	AAAATGCAAACACGGCTTGG	50.0	45.0	57.9	58.4	398-411 bp	1.0	77.2