Hotspot

ndhF

SSC · 112586-114811 · 2226 bp

Pi 0.00503

MarkerSeek score 34.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 24
pi: 0.00503
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.0	57.9	2610-2752 bp	1.0	84.6
ndhF_p2	AAGAGGGATCCACCGAAGAA	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.0	57.9	2602-2744 bp	1.0	84.6
ndhF_p3	CCTTCCCCTTCCCTTTGTTC	AGAGCTGGTGAATCGGAAAC	55.0	50.0	58.1	57.9	2579-2721 bp	1.0	84.4
ndhF_p4	TTTAGGCAGAATACCGTCGC	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.1	57.9	2712-2854 bp	1.0	84.2
ndhF_p5	GGGGACTGAACAGGAACAAG	AGAGCTGGTGAATCGGAAAC	55.0	50.0	58.1	57.9	2619-2761 bp	1.0	84.1