Hotspot

ndhG-ndhI

SSC · 121327-121741 · 415 bp

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Pi 0.0306
MarkerSeek score 66.20
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
415
variable_sites
26
pi
0.0306
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 GTGCAGCAGCTACAAAATGG ACGGGCTGCAAGGTATATTG 50.0 50.0 58.0 58.0 1113-1143 bp 1.0 83.7
ndhG-ndhI_p2 GTGCAGCAGCTACAAAATGG TGGTCAACAAACAGTACGGG 50.0 50.0 58.0 58.0 1128-1158 bp 1.0 83.5
ndhG-ndhI_p3 TCCCAACGAAAAGGCAGAAA ACGGGCTGCAAGGTATATTG 45.0 50.0 57.9 58.0 1049-1079 bp 1.0 83.5
ndhG-ndhI_p4 TCCCAACGAAAAGGCAGAAA TGGTCAACAAACAGTACGGG 45.0 50.0 57.9 58.0 1064-1094 bp 1.0 83.3
ndhG-ndhI_p5 GTGCAGCAGCTACAAAATGG ACGAGATGTTCCCCATGGTA 50.0 50.0 58.0 58.1 1165-1195 bp 1.0 82.7