Hotspot

trnN-GUU-ndhF

IRb · 111070-112585 · 1516 bp

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Pi 0.0157
MarkerSeek score 60.40
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
IRb
length_bp
1516
variable_sites
46
pi
0.0157
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 TCGGAATAGCGGACCAAATG ATCTAGGTGGAGGGCGTATC 50.0 55.0 58.1 58.1 2083-2244 bp 1.0 84.9
trnN-GUU-ndhF_p2 TCAAAGCGAGCCCTTTCATT ATCTAGGTGGAGGGCGTATC 45.0 55.0 58.1 58.1 1630-1790 bp 1.0 84.9
trnN-GUU-ndhF_p3 GCCATGGAAGAATCGGCATA ATCTAGGTGGAGGGCGTATC 50.0 55.0 58.1 58.1 2011-2171 bp 1.0 84.5
trnN-GUU-ndhF_p4 TATGCCATGGAAGAATCGGC ATCTAGGTGGAGGGCGTATC 50.0 55.0 58.1 58.1 2014-2174 bp 1.0 84.5
trnN-GUU-ndhF_p5 GGAACGGGTTACCTACTCCT ATCTAGGTGGAGGGCGTATC 55.0 55.0 58.1 58.1 1655-1815 bp 1.0 84.5