Hotspot

psbT-pbf1

LSC · 76311-76387 · 77 bp

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Pi 0.0584
MarkerSeek score 65.10
Primer available yes
Label psbT-pbf1

Feature Metadata

label_name
psbT-pbf1
feature_type
igs
region
LSC
length_bp
77
variable_sites
8
pi
0.0584
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-pbf1_p1 TATGGTGGCGAACTTGATGG CGACGGTTTGTGTAGCCATA 50.0 50.0 58.0 58.0 939 bp 1.0 81.4
psbT-pbf1_p2 ACACATTTGGCATGGTGCTA CGACGGTTTGTGTAGCCATA 45.0 50.0 58.1 58.0 748 bp 1.0 81.4
psbT-pbf1_p3 TATGGTGGCGAACTTGATGG CTAGAACTACCCTCGACGGT 50.0 55.0 58.0 58.0 952 bp 1.0 81.1
psbT-pbf1_p4 TATGGTGGCGAACTTGATGG TTCCATCCAGCAAAACGGAA 50.0 45.0 58.0 58.0 1128 bp 1.0 81.0
psbT-pbf1_p5 GCGAACTTGATGGAGTCAGT CGACGGTTTGTGTAGCCATA 50.0 50.0 57.9 58.0 932 bp 1.0 81.0