Hotspot

rps12-clpP

LSC · 71703-71998 · 296 bp

Back to Adonis

Pi 0.0316
MarkerSeek score 69.00
Primer available yes
Label rps12-clpP

Feature Metadata

label_name
rps12-clpP
feature_type
igs
region
LSC
length_bp
296
variable_sites
18
pi
0.0316
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.90
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP_p1 AGAGCGGGGGATTTTGTTAC TATGTCAGCAACAGAAGCCC 50.0 50.0 57.9 57.9 395-463 bp 1.0 85.5
rps12-clpP_p2 AGAGCGGGGGATTTTGTTAC CTTGTAGCGGTCGAAAATGC 50.0 50.0 57.9 57.8 355-423 bp 1.0 85.5
rps12-clpP_p3 GAGCGGGGGATTTTGTTACA TATGTCAGCAACAGAAGCCC 50.0 50.0 58.2 57.9 394-462 bp 1.0 85.1
rps12-clpP_p4 GAGCGGGGGATTTTGTTACA CTTGTAGCGGTCGAAAATGC 50.0 50.0 58.2 57.8 354-422 bp 1.0 85.1
rps12-clpP_p5 AGAGCGGGGGATTTTGTTAC GAAAATGCCGGGGATTTCAC 50.0 50.0 57.9 57.7 343-411 bp 1.0 84.5