Hotspot

ndhC-trnV-UAC

LSC · 52822-54206 · 1385 bp

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Pi 0.019
MarkerSeek score 70.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1385
variable_sites
48
pi
0.019
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCTTCGTTAATTGGGGCCAA GGTTCGAATCCGTATAGCCC 50.0 55.0 58.4 57.9 1460-1517 bp 1.0 81.9
ndhC-trnV-UAC_p2 TAATTGGGGCCAAAACTCCG GGTTCGAATCCGTATAGCCC 50.0 55.0 58.4 57.9 1453-1510 bp 1.0 81.9
ndhC-trnV-UAC_p3 TGGTCCTTCGTTAATTGGGG GGTTCGAATCCGTATAGCCC 50.0 55.0 57.5 57.9 1464-1521 bp 1.0 81.5
ndhC-trnV-UAC_p4 CCTTCGTTAATTGGGGCCAA CGGTTCGAATCCGTATAGCC 50.0 55.0 58.4 58.3 1461-1518 bp 1.0 80.1
ndhC-trnV-UAC_p5 TAATTGGGGCCAAAACTCCG CGGTTCGAATCCGTATAGCC 50.0 55.0 58.4 58.3 1454-1511 bp 1.0 80.1