Hotspot

trnD-GUC-psbM

LSC · 21840-22431 · 592 bp

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Pi 0.00676
MarkerSeek score 60.50
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
592
variable_sites
8
pi
0.00676
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TTCAATTGGTCAGAGCACCG TGCGAGAATATTGACTTCCA 50.0 40.0 58.5 54.5 677-686 bp 1.0 50.7
trnD-GUC-psbM_p2 TTCAATTGGTCAGAGCACCG ATGCGAGAATATTGACTTCCA 50.0 38.1 58.5 55.1 678-687 bp 1.0 47.7
trnD-GUC-psbM_p3 TTCAATTGGTCAGAGCACCG TGCGAGAATATTGACTTCCAT 50.0 38.1 58.5 55.1 677-686 bp 1.0 47.7
trnD-GUC-psbM_p4 GTTCAATTGGTCAGAGCACC TGCGAGAATATTGACTTCCA 50.0 40.0 57.0 54.5 678-687 bp 1.0 46.6
trnD-GUC-psbM_p5 GCATGCCTTATTTCTCTGGGA TGCGAGAATATTGACTTCCA 47.6 40.0 58.1 54.5 704-713 bp 1.0 45.9