Hotspot

trnY-GUA-trnD-GUC

LSC · 21353-21765 · 413 bp

Back to Adonis

Pi 0.0295
MarkerSeek score 68.10
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
413
variable_sites
24
pi
0.0295
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 TGGCAATATGTCTACGCTGG TACTAAGTCGGGACTGACGG 50.0 55.0 57.8 58.3 535-558 bp 1.0 80.3
trnY-GUA-trnD-GUC_p2 GGCAATATGTCTACGCTGGT TACTAAGTCGGGACTGACGG 50.0 55.0 57.8 58.3 534-557 bp 1.0 80.3
trnY-GUA-trnD-GUC_p3 GGGGACGGACTGTAAATTCG TACTAAGTCGGGACTGACGG 55.0 55.0 58.4 58.3 556-579 bp 1.0 79.3
trnY-GUA-trnD-GUC_p4 GGGACGGACTGTAAATTCGT TACTAAGTCGGGACTGACGG 50.0 55.0 57.6 58.3 555-578 bp 1.0 79.1
trnY-GUA-trnD-GUC_p5 TGGCAATATGTCTACGCTGG CGGTGCTCTGACCAATTGAA 50.0 50.0 57.8 58.5 483-506 bp 1.0 79.0